Biological Sensors
Automated Toxin Detection System (412)
Problem: Need an instrument system to provide rapid, sensitive detection of toxins in complex matrixes.
Project Activity Scope (from Technical Overview Chart): Sensors and Devices through Integrated Systems
PNNL Solution:
- Automated system based on renewable microbead column
- Affinity matured antibodies (IgG) give high sensitivity and selectivity
- Complex samples can be rapidly processed and target species extracted
- Highly sensitive on-column fluorescence detection using customized optics/detector
- Successfully demonstrated
Capabilities Employed to Meet Client Needs: Surface chemistry, enzyme chemistry, detector physics, electronics, micro fluidics control, electronics systems, quantitative analysis, fluorescent nanoparticle (quantum dot) synthesis and characterization, quantum dot conjugation and attachment, fluorescence spectroscopy, laser detection, custom optics and fiber optics, affinity-matured antibody development, stabilized enzymes and antibodies, single chain antibodies, custom protein synthesis, non-immunologically derived capture proteins, combinatorial chemistry, Biacore testing.
Selected Links to Related Information:
- Related Examples (Same Project)
- BEADS: A Basic Tool for Bio-Detection (255)
- Renewable Chem-Bio Sensor System (409)
- Automated BEAD Suspension Arrays (254)
- Automated Fluidics for BEADS Sensors (411)
- BEADS Automated Immunoassays (259)
- BEADS: Automated Sample Processing System for Biodetection (260)
- BEADS for Separations, Processing, Sensing (261)
- BEADS for Selective Analyte Capture (407)
- Nucleic Acid Extraction and Purification (408)
- "Reporters" Indicate Target Analyte Capture (410)
- Related Websites
- Breakthroughs, Winter/Spring 2002 (.pdf 7.3MB - see second item)
- BEADS (Biodetection Enabling Analyte Delivery System) Sample Preparation Technology
