Biological Sensors
"Reporters" Indicate Target Analyte Capture (410)
Problem: Need stable, reliable methods to detect and quantify the capture of target analyte species on test beads.
Project Activity Scope (from Technical Overview Chart): Mechanisms through Sensors and Devices
PNNL Solution:
- Attach fluorescent tags to tether species that will indicate target analyte capture
- Employ fluorescent nanoparticles ("quantum dots") captured with target analyte
- Characterize excitation and emission characteristics for target analyte and possible confounders
- Validate performance for selected target analytes
Capabilities Employed to Meet Client Needs: Surface chemistry, enzyme chemistry, detector physics, electronics, micro fluidics control, electronics systems, quantitative analysis, fluorescent nanoparticle (quantum dot) synthesis and characterization, quantum dot conjugation and attachment, fluorescence spectroscopy, time-resolved fluorescence, time-correlated single-photon counting (TCSPC).
Selected Links to Related Information:
- Related Examples (Same Project)
- Similar Application Examples (Same or Different Technology)
- Similar Technology Examples (Same or Different Application)
- Related Websites
- Breakthroughs, Winter/Spring 2002 (.pdf 7.3MB - see second item)
- BEADS (Biodetection Enabling Analyte Delivery System) Sample Preparation Technology
