Biological Sensors
Proteomics for Biomarker Identification (253)
Problem: Find high specificity biomarkers for pathogen identification to enable development of sensors and antibodies
Project Activity Scope (from Technical Overview Chart): Mechanisms through Instruments
PNNL Solution:
- Use state-of-the-art FTICR-MS
- Use PNNL Global Proteomics capability to identify all the proteins from an organism across different culture conditions
- Perform comparative analysis of pathogenic versus non-pathogenic organisms for unique biomarkers and use results to create organism peptide identification libraries
Capabilities Employed to Meet Client Needs: Fourier Transform – Ion Cyclotron Resonance (FTICR) – mass spectrometry user facility within PNNL-EMSL; protein extraction protocols; ultra high pressure high performance liquid chromatography separations; Sequest database searching; high speed data extraction and analysis algorithms.
Selected Links to Related Information:
- Similar Application Examples (Same or Different Technology)
Contact: Mary Lipton
