Sensors & Electronics
Biological Sensors
Two-Color Array Method for Stress Analysis (448)
Problem: Measure environmental stress effect on messenger RNA population (+ or -) with minimal arrays and analysis.
Project Activity Scope (from Technical Overview Chart): Mechanisms through Sensors and Devices.
PNNL Solution:
- Labeled control and test mRNA fragments with different color fluorescent tags
- Prepared one mRNA array
- Mixed solutions and applied to array
- Dominant fragment population for each array pixel determined pixel color
- Pixel color indicated stress effects
Capabilities Employed to Meet Client Needs: Oligonucleotide dye labeling, array hybridization and data analysis.
Selected Links to Related Information:
- Related Examples (Same Project)
- Alternate Methods to Monitor Stress Effects (446)
- Concept for Two-Color Array Mechanism (449)
- Conceptual Introduction to DNA Basics (450)
- DNA Array Basics (451)
- Detect Early Effects of Environmental Stress (444)
- Determine Sensitive Stress Indicator (445)
- Dual Array Method for Stress Analysis (447)
- Organism Identification from Array Response (459)
- Tailored DNA Detector Array Preparation (457)
- Similar Technology Examples (Same or Different Application)
- Related Websites
- Microarrays: Chipping Away at the Mysteries of Science and Medicine — A Science Primer, National Center for Biotechnology Information, US National Institutes of Health, July 2007
Contact: Tim Straub
