Sensors & Electronics
Biological Sensors
Concept for Two-Color Array Mechanism (449)
Problem: Label mRNA with distinctive color tags to allow two-color array assessment of species response under stress.
Project Activity Scope (from Technical Overview Chart): Mechanisms through Sensors and Devices.
PNNL Solution:
- Labeled control and tested mRNA with different color fluorescent tags [e.g., control = green and test = red]
- When mRNA was combined, color of dominant population determined color of individual array elements
- Array color pattern indicated species response to stressor
Capabilities Employed to Meet Client Needs: Oligonucleotide dye labeling, array hybridization and data analysis.
Selected Links to Related Information:
- Related Examples (Same Project)
- Alternate Methods to Monitor Stress Effects (446)
- Conceptual Introduction to DNA Basics (450)
- Detect Early Effects of Environmental Stress (444)
- Determine Sensitive Stress Indicator (445)
- DNA Array Basics (451)
- Dual Array Method for Stress Analysis (447)
- Two-Color Array Method for Stress Analysis (448)
- Similar Application Examples (Same or Different Technology)
Contact: Tim Straub
