Sensors & Electronics
Biological Sensors
Broad Response DNA Detector Arrays (458)
Problem: Need to detect and identify unknown microorganisms in water with a low number of tests (unconstrained threats).
Project Activity Scope (from Technical Overview Chart): Mechanisms through Sensors and Devices.
PNNL Solution:
- Prepared sensor arrays with broad range of tethered DNA fragments (able to capture fragments from a variety of relevant threat organisms)
- Labeled probes from the community of DNA signatures in the sample were applied to the array
- Recorded array response pattern after exposure to water sample
Capabilities Employed to Meet Client Needs: Nucleic acid extraction, oligonucleotide design, array printing, array hybridization and detection.
Selected Links to Related Information:
- Related Examples (Same Project)
- Alternate Methods for Organism Detection (455)
- Concentrate Microorganisms for Analysis (453)
- Conceptual Introduction to DNA Basics (450)
- DNA Array Basics (451)
- Organism Identification from Array Response (459)
- Organism Specific DNA Detector Arrays (456)
- Tailored DNA Detector Array Preparation (457)
- Very Selective Detection with DNA Arrays (454)
- Water Screening for Microorganism Hazards (452)
- Similar Application Examples (Same or Different Technology)
- Related Websites
- Microarrays: Chipping Away at the Mysteries of Science and Medicine — A Science Primer, National Center for Biotechnology Information, US National Institutes of Health, July 2007
Contact: Tim Straub
