Sensors & Electronics
Biological Sensors
Alternate Methods for Organism Detection (455)
Problem: Determine preferred DNA/mRNA measurement method for evaluating environmental exposure effects.
Project Activity Scope (from Technical Overview Chart): Interaction Mechanisms through Sensors and Devices through Instruments.
PNNL Solution:
Two technical approaches can be used to detect microorganisms
- Used multiple, single organism arrays to detect specific organisms in sample mix
- Used broad response array to detect fragments of many organisms; interpretation depends on pattern recognition based on prior response experience
Capabilities Employed to Meet Client Needs: Nucleic acid extraction, oligonucleotide design, array printing, and array hybridization and detection.
Selected Links to Related Information:
- Related Examples (Same Project)
- Broad Response DNA Detector Arrays (458)
- Concentrate Microorganisms for Analysis (453)
- Conceptual Introduction to DNA Basics (450)
- DNA Array Basics (451)
- Organism Identification from Array Response (459)
- Organism Specific DNA Detector Arrays (456)
- Tailored DNA Detector Array Preparation (457)
- Very Selective Detection with DNA Arrays (454)
- Water Screening for Microorganism Hazards (452)
- Similar Application Examples (Same or Different Technology)
Contact: Tim Straub
