Sensors & Electronics
Biological Sensors
Very Selective Detection with DNA Arrays (454)
Problem: Need extremely selective microorganism capture and detection to ensure proper organism identification
Project Activity Scope (from Technical Overview Chart): Mechanisms through Sensors and Devices.
PNNL Solution:
- Exploited extremely selective binding between matching DNA fragments
- Tethered selected DNA fragments to array plate to capture specific DNA fragments from the sample
- Perfectly matched fragments bind tightly
- Poorly matched fragments don’t bind and rinse off array
Capabilities Employed to Meet Client Needs: Nucleic acid extraction, oligonucleotide design, array printing, array hybridization and detection.
Selected Links to Related Information:
- Related Examples (Same Project)
- Alternate Methods for Organism Detection (455)
- Broad Response DNA Detector Arrays (458)
- Concentrate Microorganisms for Analysis (453)
- Conceptual Introduction to DNA Basics (450)
- DNA Array Basics (451)
- Organism Specific DNA Detector Arrays (456)
- Organism Identification from Array Response (459)
- Tailored DNA Detector Array Preparation (457)
- Water Screening for Microorganism Hazards (452)
- Similar Application Examples (Same or Different Technology)
- Related Websites
- Microarrays: Chipping Away at the Mysteries of Science and Medicine — A Science Primer, National Center for Biotechnology Information, US National Institutes of Health, July 2007
Contact: Tim Straub
