Sensors & Electronics
Biological Sensors
Organism Specific DNA Detector Arrays (456)
Problem: Need reliable, highly specific water screening to detect a specific microorganism in water samples.
Project Activity Scope (from Technical Overview Chart): Mechanisms through Sensors and Devices.
PNNL Solution:
- Identified target organism's "signature" DNA fragments
- Considered possible confounding signatures from co-present organisms
- Prepared organism specific detector array
- Additional arrays required for other target organisms
- Not optimal for "unknowns"
Capabilities Employed to Meet Client Needs: Nucleic acid extraction, oligonucleotide design, array printing, array optimization, and array hybridization and detection.
Selected Links to Related Information:
- Related Examples (Same Project)
- Alternate Methods for Organism Detection (455)
- Broad Response DNA Detector Arrays (458)
- Conceptual Introduction to DNA Basics (450)
- Concentrate Microorganisms for Analysis (453)
- DNA Array Basics (451)
- Organism Identification from Array Response (459)
- Tailored DNA Detector Array Preparation (457)
- Very Selective Detection with DNA Arrays (454)
- Water Screening for Microorganism Hazards (452)
- Similar Technology Examples (Same or Different Application)
- Related Websites
- Microarrays: Chipping Away at the Mysteries of Science and Medicine — A Science Primer, National Center for Biotechnology Information, US National Institutes of Health, July 2007
Contact: Tim Straub
