Biological Sensors
Biofilm Characterization/Measurements (378)
Problem: Need effective methods to characterize and quantify biologically important properties of biofilms.
Project Activity Scope (from Technical Overview Chart): Interaction Mechanisms through Measurement and Analysis Method Development
PNNL Solution:
- Acoustic microscopy can provide structural and material property data.
- Time-domain analysis of data provided 3-D images of biofilms.
- Estimated film thickness in good agreement with optical methods.
- Frequency domain analysis provided thickness, density and elastic stiffness information.
Capabilities Employed to Meet Client Needs: Acoustic microscope; high frequency ultrasonic measurement; mechanical scanning system with 1 µm resolution; time-resolved and frequency-resolved image analysis; statistical analysis; state-of-art scattering models to infer internal structure; nm sized ultrasonic contrast agents; verification using confocal laser scanning microscope; cultivation of repeatable biofilm specimens with improved Constant Depth Film Fermenter (CDFF).
Selected Links to Related Information:
- Similar Technology Examples (Same or Different Application)
Contact: Salahuddin Ahmed
