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Sensors & Electronics

Biological Sensors

Detecting Bacteria in Powders (331)

Problem: Need practical measurement method for rapid, non-invasive detection and identification of bacteria

Representative second-derivative spectra of the five strains (sporulated forms) analyzed for the reference library showing the subtle differences among strains

Project Activity Scope (from Technical Overview Chart): Mechanisms through Sensors and Devices

PNNL Solution:

  • Use photoacoustic spectroscopy (PAS) for direct analysis of dry samples
  • Detect bacteria using infrared absorption features identified in spectral library for bacteria
  • Adapt laser source(s) and PAS cell design to allow practical field-portable bacterial analysis

Capabilities Employed to Meet Client Needs: Spectroscopic understanding of molecules and bacteria; use of electronics to collect data; data processing and analysis; understanding and operation of FT-infrared spectrometers for spectra characterization; microbiological techniques in cell culturing; statistical analysis of large, complex data sets, data analysis to obtain key chemical and biological information; reporting of findings (including numerous open literature publications).

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Contact: Nancy Foster-Mills

Sensors & Electronics

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