Biological Sensors
Rapid Antibody Production Using Yeast (231)
Problem: Rapidly and efficiently find and produce high specificity antibodies in order to allow timely response to new threats.
Project Activity Scope (from Technical Overview Chart): Mechanisms through Sensors and Devices
PNNL Solution:
- Identify antibody on a yeast clone with specificity to the antigen of interest from a library of 109 possible antibodies in the yeast population
- Screen population containing the desired antibody specificity using flow cytometry
- Utilized for microarray detector at sub pg/ml to detect Botulinum toxin KD 30pM
- Capability resulted in R&D 100 Award
Capabilities Employed to Meet Client Needs: Biotechnology; antibody engineering; high speed cell sorting; antibody library development.
Selected Links to Related Information:
- Related Websites
Contact: Cheryl Baird
