Biological Sensors
Rapid Extraction of Spore Biomarkers (239)
Problem: Develop a method to rapidly extract exploitable biomarkers from difficult to penetrate bacterial endospores
Project Activity Scope (from Technical Overview Chart): Mechanisms through Sensors and Devices
PNNL Solution:
- Use supercritical fluid extraction (SFE) methods to rapidly extract usable biomarkers from spore shell without requiring cell lysing
- Adapt to PNNL’s field portable SFE device
- Low consumables and rapid extraction times
- Interface extraction process to selective absorbent for sample preparation and analysis processes
- Unique biomarkers can be easily identified using MALDI-MS and/or fluorescence arrays
Capabilities Employed to Meet Client Needs: Supercritical fluid expertise including methods and development of field portable equipment; spore preparation expertise; MALDI-MS capability for detection of lipid, peptide and protein biomarkers; GC-MS capability for detection of volatile biomarkers.
Selected Links to Related Information:
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Contact: Jon Wahl
